These protocols provide a materials list and step-by-step instructions for the extraction, preparation, isolation, and purification of proteins. Topics of the 44 chapters include purification and concentration by ultrafiltration, hydrophobic interaction chromatography, affinity precipitation methods, removal of detergent from protein fractions, storage of pure proteins, and electroblotting of proteins from poly-acrylamide gels. The second edition adds chapters on protein fractionation and chromatographic techniques Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)