Recent advances in the field of fluorescence microscopy has made it an invaluable tool for biological and biomedical science and cell imaging. This helpful guide brings together experts in their field to offer clear reproducible techniques for the best utilization of microscopy. Smalls tips and tricks not covered in primary literature are designed to both instruct and inspire the reader to try novel approaches. Super-resolution techniques, light sheet microscopy, sample preparation, labeling and analysis are all covered in detail. A companion website includes video accompaniments of challenging techniques. Annotation ©2014 Ringgold, Inc., Portland, OR (protoview.com)