This collection of 18 new articles covers current and emerging methods of designing vectors for gene delivery and expression. Focusing on efficient, targeted and regulated delivery, the contributors of these papers give hints and tricks to ensure success as they describe the purification of retrovirus particles using heparin affinity chromatography, the quantification of the titer and quality of adenovirus stocks, real time multiple particle tracking or curve nanocarriers in complex biological environments, production of lentiviruses displaying early-acting cytokines for selective gene transfer into hematipoietic stem cells, photochemical enhancement of DNA delivery by EGF receptor target polyplexes, evaluation of promoters for use in tissue-specific gene delivery, liver-directed gene therapy using the Sleeping Beauty system, and conditional gene expression and knockdown using lentivirus vectors encoding shRNA. Annotation ©2008 Book News, Inc., Portland, OR (booknews.com)