Metabonomics — defined as the quantitative measurement of the multiparametric, time-related metabolic responses of a complex (multicellular) system to a pathophysiological intervention or genetic modification — is an effective tool for toxicological studies, potential biomarker identification of diseases, and identification of regulatory network pathways in plants and animals. Metabonomics is discussed in detail in many applications such as drug development, disease treatment and modernization of traditional Chinese medicine. This book covers research on metabonomics, ranging from the development of specialized chemical analytical techniques to the construction of databases and methods for metabolic simulation. The authors have been directly involved in the development of all the subject areas, including gas chromatography, liquid chromatography, mass spectrometry, metabolic databases, and metabolic simulation. Basic definition, breakthrough achievements and the future of metabonomic studies are described, making this book a valuable source for researchers in metabonomics in diverse fields such as animal, cellular, microbial, pharmaceutical, medical, and life sciences.