In this volume, experts describe in detail their best techniques for the isolation, culture and study of both activation and signaling in human mast cells. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells; the development of mast cells in vitro; the study of mast cell signaling and gene expression; and the measurement of mast cell expression of inflammatory mediators. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. Step-by-step laboratory instructions, an outline of principles, lists of necessary equipment and reagents, and troubleshooting tips are included for the protocols. Annotation ©2005 Book News, Inc., Portland, OR (booknews.com)