The laboratory mouse is an important model for studying cancer and its treatment. This book includes both background information and step-by-step protocols for generating mouse models that accurately recapitulate many features of human cancer. It covers genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using chemical carcinogens, RNA interference, tissue recombination, and other strategies. Techniques to characterize tumor development, progression, and metastasis in these mice using state-of-the-art imaging, histopathological, surgical, and other approaches are described. The uses of these models in preclinical and translational research are also discussed.