<p>This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, <i>in silico</i> PCR primer design, and primer design using software. Written in the highly successful <i>Methods in Molecular Biology </i>series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p>Authoritative and easily accessible, <i>PCR Primer Design, Second Edition</i> seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.</p>